| Module-I
Instrumentation Systems- |
DNA Sequencing Technologies- PCR, Realtime PCR, PCR-DGGE, Sanger sequencing,
Fragment sequencing, Multiplex SNP, typing, Next Gen Sequencing
|
1 week
|
| Module-II
Human Identification (HID) |
Laboratory Experiments (hands on): DNA isolation from human samples and
quantification, PCR amplification using multiplex primers, fragment (allele)
separation following automated capillary electrophoresis, data capturing, data
analysis and DNA fingerprint development.
|
2 weeks
|
| Module -III
DNA Barcoding and Genetic Diversity |
Laboratory Experiments (hands on): DNA isolation from bio-resource, quantification
Specific specific/Breed specific PCR using universal, multiplex primers following by
automated DNA sequencing, data capturing, data analysis.
|
3 weeks
|
| Module –IV
DNA based Disease diagnosis |
DNA isolations from patients samples (Blood/sputum/serum), DNA quantification,
allele specific PCR in disease diagnosis/identification of pathogens, pathogen
specific PCR, PCR-RFLP, Identification of viral load, Gene expression studies by
Realtime PCR.
|
2 weeks
|
| Module-V
Bioinformatics |
Primer Designing, Trace file analysis (Sequence raw file), BLAST, Sequence alignment
annotation, Phylogenetic Tree construction, Genetic Distance calculation,
Sequence Sequence Submission to NCBI.
|
1 week |
